The Year in CRISPRs - 15

Using CRISPR
as a High-Throughput Cancer
Screening and Modeling Tool
A

contingent of researchers from the U.K.,
Germany, and Spain have recently
developed a novel CRISPR-Cas9 system that
they believe can be utilized as a multiplexed
screening approach to study and model cancer
development in mice. In the current study, the
investigators directly mutated genes within adult
mouse livers to elucidate their role in cancer
development and progression-simultaneously
uncovering the gene combinations that
coordinate to cause liver cancer.

15

| GENengnews.com

"We reasoned that, by targeting mutations
directly to adult liver cells using CRISPR-Cas9,
we could better study and understand the
biology of this important cancer," explained
co-author Mathias Friedrich, Ph.D., research
scientist at the Wellcome Trust Sanger Institute.
"Other approaches to engineer mutations in mice,
such as stem cell manipulation, are limited by the
laborious process, the long time frames and large
numbers of animals needed. And, our method
better mimics important aspects of human
cancer biology than many "classic" mouse
models: as in most human cancers, the mutations
occur in the adult and only affect a few cells."

The findings from this study were published
online recently in PNAS through an article
entitled "CRISPR-Cas9 somatic multiplexmutagenesis for high-throughput functional
cancer genomics in mice."
This new approach is rapid, scalable, and
extremely efficient, allowing the researchers to
examine an array of genes or large regions of
the genome concurrently. Moreover, this
methodology affords scientists the ability to
distinguish between cancer driver mutations
and passenger mutations-those that occur
as side-effects of cancer development.


http://www.GENengnews.com

The Year in CRISPRs

Table of Contents for the Digital Edition of The Year in CRISPRs

Contents
The Year in CRISPRs - 1
The Year in CRISPRs - 2
The Year in CRISPRs - 3
The Year in CRISPRs - Contents
The Year in CRISPRs - 5
The Year in CRISPRs - 6
The Year in CRISPRs - 7
The Year in CRISPRs - 8
The Year in CRISPRs - 9
The Year in CRISPRs - 10
The Year in CRISPRs - 11
The Year in CRISPRs - 12
The Year in CRISPRs - 13
The Year in CRISPRs - 14
The Year in CRISPRs - 15
The Year in CRISPRs - 16
The Year in CRISPRs - 17
The Year in CRISPRs - 18
The Year in CRISPRs - 19
The Year in CRISPRs - 20
The Year in CRISPRs - 21
https://www.nxtbookmedia.com