The Year in CRISPRs - 8

"Targeting of the acetyltransferase shows that gene activation is
highly specific," the authors wrote in a prepublication version of their
article. "In contrast to previous dCas9-based activators, the acetyltransferase
activates genes from enhancer regions and with only one guide RNA."

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The Year in CRISPRs * CRISPR-Cas9 Edits the Epigenome

The scientists, led by Charles Gersbach, Ph.D., assistant professor of
biomedical engineering at Duke, noted that their epigenome-editing
technology will advance several lines of investigation. It will help researchers
explore the roles that particular promoters and enhancers play in cell fate
or the risk for genetic disease. In addition, it could provide a new avenue
for gene therapies and guiding stem cell differentiation.
"There are already drugs that will affect enhancers across the whole
genome, but that's like scorching the earth," said Timothy Reddy, Ph.D.,
assistant professor of biostatistics and bioinformatics at Duke. "I wanted
to develop tools to go in and modify very specific epigenetic marks in
very specific places to find out what individual enhancers are doing."
Dr. Reddy found that specificity by teaming up with Dr. Gersbach, his
neighbor within Duke's Center for Genomic and Computational Biology.
"It's like we use CRISPR to find a genetic address so that we can alter the
DNA's packaging at that specific site," said Dr. Reddy.

8

| GENengnews.com

Scientists at Duke University chemically altered histones to turn enhancers on
in order to gain a better understanding of the role enhancers play in impacting
genetic disease.


http://www.GENengnews.com

The Year in CRISPRs

Table of Contents for the Digital Edition of The Year in CRISPRs

Contents
The Year in CRISPRs - 1
The Year in CRISPRs - 2
The Year in CRISPRs - 3
The Year in CRISPRs - Contents
The Year in CRISPRs - 5
The Year in CRISPRs - 6
The Year in CRISPRs - 7
The Year in CRISPRs - 8
The Year in CRISPRs - 9
The Year in CRISPRs - 10
The Year in CRISPRs - 11
The Year in CRISPRs - 12
The Year in CRISPRs - 13
The Year in CRISPRs - 14
The Year in CRISPRs - 15
The Year in CRISPRs - 16
The Year in CRISPRs - 17
The Year in CRISPRs - 18
The Year in CRISPRs - 19
The Year in CRISPRs - 20
The Year in CRISPRs - 21
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