# Facing the Challenges in Vaccine Upstream Bioprocessing - 26

```Facing the Challenges in Vaccine Upstream Bioprocessing * rAAV Production in Suspension CAP GT® Cells in BioBLU® 3c and 10c Single-Use Vessels

Power number
The impeller power number is a dimensionless
number associated with different types of
impellers. The impeller power number is used
to calculate P/V using the following equation:
P/V = (Np x ρ x N³ x d5)/V
with Np being the impeller power number,
ρ being the density of water (1000 kg/m³),
N being the agitation speed (rps), d being the
impeller outer diameter (m), and V being the
full working volume (m³). To calculate P/V, the
bioprocess engineers at Cevec used impeller
power numbers which were experimentally
determined in the Eppendorf applications
laboratory. Experimental determination of
power numbers using a rotational torque sensor
requires a direct drive motor. To be able to
measure the power numbers for BioBLU SingleUse Vessels-which have a magnetic drive-,
the Eppendorf application scientists modified
the vessels to remove the magnet from the
magnetic drive coupling. They then connected
the torque sensor directly to the impeller shaft.
26

| GENengnews.com

Figure 2. Power numbers of BioBLU c Single-Use Vessels with one impeller were determined
according to [1] and [2].
Power numbers were measured at tip speeds
between 0.3 and 1 m/s and then the average
was calculated. For the BioBLU 3c the mean was
2.98 and for the BioBLU 10c the mean was 3.33
(Figure 2).

```
http://www.GENengnews.com

# Facing the Challenges in Vaccine Upstream Bioprocessing

Contents
Facing the Challenges in Vaccine Upstream Bioprocessing - 1
Facing the Challenges in Vaccine Upstream Bioprocessing - 2
Facing the Challenges in Vaccine Upstream Bioprocessing - 3
Facing the Challenges in Vaccine Upstream Bioprocessing - Contents
Facing the Challenges in Vaccine Upstream Bioprocessing - 5
Facing the Challenges in Vaccine Upstream Bioprocessing - 6
Facing the Challenges in Vaccine Upstream Bioprocessing - 7
Facing the Challenges in Vaccine Upstream Bioprocessing - 8
Facing the Challenges in Vaccine Upstream Bioprocessing - 9
Facing the Challenges in Vaccine Upstream Bioprocessing - 10
Facing the Challenges in Vaccine Upstream Bioprocessing - 11
Facing the Challenges in Vaccine Upstream Bioprocessing - 12
Facing the Challenges in Vaccine Upstream Bioprocessing - 13
Facing the Challenges in Vaccine Upstream Bioprocessing - 14
Facing the Challenges in Vaccine Upstream Bioprocessing - 15
Facing the Challenges in Vaccine Upstream Bioprocessing - 16
Facing the Challenges in Vaccine Upstream Bioprocessing - 17
Facing the Challenges in Vaccine Upstream Bioprocessing - 18
Facing the Challenges in Vaccine Upstream Bioprocessing - 19
Facing the Challenges in Vaccine Upstream Bioprocessing - 20
Facing the Challenges in Vaccine Upstream Bioprocessing - 21
Facing the Challenges in Vaccine Upstream Bioprocessing - 22
Facing the Challenges in Vaccine Upstream Bioprocessing - 23
Facing the Challenges in Vaccine Upstream Bioprocessing - 24
Facing the Challenges in Vaccine Upstream Bioprocessing - 25
Facing the Challenges in Vaccine Upstream Bioprocessing - 26
Facing the Challenges in Vaccine Upstream Bioprocessing - 27
Facing the Challenges in Vaccine Upstream Bioprocessing - 28
Facing the Challenges in Vaccine Upstream Bioprocessing - 29
Facing the Challenges in Vaccine Upstream Bioprocessing - 30
Facing the Challenges in Vaccine Upstream Bioprocessing - 31
Facing the Challenges in Vaccine Upstream Bioprocessing - 32
Facing the Challenges in Vaccine Upstream Bioprocessing - 33
Facing the Challenges in Vaccine Upstream Bioprocessing - 34
Facing the Challenges in Vaccine Upstream Bioprocessing - 35
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