Facing the Challenges in Vaccine Upstream Bioprocessing * rAAV Production in Suspension CAP GT® Cells in BioBLU® 3c and 10c Single-Use Vessels A B Figure 4. A. DO control. B. pH control. with similar profiles for all four runs (Figure 4A). All four runs showed similar pH profiles within the regulation parameters (Figure 4B). The researchers determined transfection efficiency based on green fluorescent protein (GFP) fluorescence measured with a NucleoCounter NC-3000. Similar transfec28 | GENengnews.com tion efficiencies of around 70 % were achieved at both scales. The transfection efficiency was comparable to that of the original shake flask process (Figure 5). The bioprocess engineers at Cevec measured productivity by quantification of viral genome titer by qPCR. The viral genome titer was similar in the bioprocess runs with a working volume of 2 L and 10 L and again comparable to the original shake flask process (Figure 6).http://www.GENengnews.com