Facing the Challenges in Vaccine Upstream Bioprocessing * rAAV Production in Suspension CAP GT® Cells in BioBLU® 3c and 10c Single-Use Vessels Figure 5. Transfection efficiency Conclusion Bioprocess engineers at Cevec scaled-up a rAAV production process from BioBLU 3c Single-Use Vessels with a working volume of 2 L to BioBLU 10c Single-Use Vessels with a working volume of 10 L. Cell growth, transfection efficiency, and productivity were comparable at 29 | GENengnews.com Figure 6. Productivity both scales. This process development enables then the production of higher amounts of rAAV gene therapy vectors by transient transfection. Moreover, these results give an example for successful bioprocess scale-up based on constant P/V using differently sized BioBLU Single-Use Vessels. n References 1. DECHEMA Expert Group Single-Use Technology. Recommendations for process engineering characterisation of single-use bioreactors and mixing systems by using experimental methods. 2016. 2. Li B, Sha M. Scale-Up of Escherichia coli Fermentation from Small Scale to Pilot Scale Using Eppendorf Fermentation Systems. Eppendorf Application Note 306. 2016.http://www.GENengnews.com