Facing the Challenges in Vaccine Upstream Bioprocessing - 30

Vero Perfusion,
Packed-Bed Vessels Intensify

Vaccine Production

Eppendorf's Fibra-Cel and BioBLU 5p Technologies
Show Promise for High-Titer Vaccine Production
Xiaofeng (Kevin) Han, Ph.D.,
Ulrike Becken, Dr.rer.nat.,
and Ma Sha, Ph.D.

30

| GENengnews.com

The disks are made of polyester and
polypropylene fibers, which provide a threedimensional environment, offering a high
surface-to-volume ratio and protecting cells
from damaging shear forces. In combination
with a basket impeller, a packed-bed bioreactor
system is formed, which enables the exchange
of growth medium and harvest of the end
product in perfusion processes, without the
need for cell filtration.

ADDITIONAL CONTENT
Microbial expression systems are highly relevant
for the biopharmaceutical industry. Single-use
fermentors, specifically designed to meet the
needs of microbial bioprocesses, have the
potential to diminish labor costs and shorten
turn-around times.
Learn More

Art Marie/Getty Images

S

trong demand for vaccines for viral diseases requires that manufacturing techniques
become more productive, including those
based on scalable bioreactor cell culture systems. In viral vaccine production, Vero is one of
the most widely used cell lines.1 Because these
cells are anchorage dependent, they require
a growth support when they are cultivated in
stirred-tank bioreactors. For high-density Vero

cell culture processes, an attachment matrix
incorporating Fibra-Cel® disks shows promise.

https://www.eppendorf.com/fileadmin/General/Applications/Bioprocess_Landing_Page/2018_Poster_High-density-E-coli-fermentation-BioBLU3f.pdf http://www.GENengnews.com

Facing the Challenges in Vaccine Upstream Bioprocessing

Table of Contents for the Digital Edition of Facing the Challenges in Vaccine Upstream Bioprocessing