Real-Time Analysis of Advanced Cell Models | QUALITY CONTROL ASSAYS lines and culture growth characteristics. The IncuCyte system is compatible with multiple different types of cell culture vessels and can monitor multiple vessels at a time. Data consists of objective, image-based growth metrics to capture transient and time-dependent events. In order to establish quality cell cultures and improve experimental outcomes in downstream assays, various aspects of cell culture need to be tightly controlled, optimized and documented. * Growth conditions: Prior to utilizing cells in quantitative assays, it is important to optimize and define cell culture regimens. Documenting variations in cell growth due to factors such as; lot-to-lot differences in cell culture media, changes in media component concentrations over time due to degradation, and inconsistent feeding schedules can all be identified using label-free phase image segmentation techniques that generate confluence measurements. A B C * Documentation of cell morphology: Changes in cell morphology due to cell seeding density or phenotypic drift can be identified using a range of magnifications to capture fine details of cells and spatial coverage of cell populations. * Accurate assessment of overall monolayer: By imaging multiple areas of your vessel or using a whole-well imaging mode, spatial variations in cell distribution can be quantified and then reduced. * Cell seeding densities: To improve assay quality 12 | January, 2019 Figure 2. T-flask vessel view shows location of images acquired (A). Proliferation time-course for HT-1080 human fibrosarcoma cells is shown (B). (C) HD-phase time-lapse images of HT-1080 cells (confluence mask overlaid; right panels).https://www.essenbioscience.com/en/