Real-Time Analysis of Advanced Cell Models | ADVANCED IN VITRO MODELING The BT-474 multi-spheroids appeared more sensitive to Herceptin cytotoxicity with the highest test concentration of Herceptin (1 µg/ml-1) producing approximately 80% inhibition compared with approximately 50% in SKOV3 multi-spheroids. the impact of stromal cells on tumor multi-spheroid resistance to chemotherapeutic agents. Brightfield in combination with fluorescence imaging permits visualization and quantification of immune cell-mediated toxicity within tumor multi-spheroids. Conclusion The IncuCyte S3 Live-Cell Analysis System enables the analysis of spheroid biology in real-time. Brightfield, in combination with phase-contrast imaging allows for labelfree assessment of changes in multi-spheroid morphology and size. With no need for selection of a predefined end-point, consistent segmentation and quantification of brightfield images enables kinetic assessment of cell-dependent growth profiles and More advanced 3D models that incorporate an ECM and additional cell types have the potential to provide more relevant translational models for the study of the tumor micro-environment on tumor biology. n Figure 5. Figure 5. Perform ADCC immune cell killing of HER2 positive multi-spheroids. PBMCs were cocultured with tumor multi-spheroids either stably expressing nuclear restricted RFP (SKOV3NR, MCF7-NR) or cytoplasmically restricted GFP (BT-474-CyG). Images show effect of Herceptin on spheroid proliferation in presence and absence of PBMCs. IncuCyte S3 brightfield object 19 | January, 2019 outline mask shown in yellow. Death quantified in real time as a loss of fluorescence intensity within the spheroid BF object. Concentration response curves to Herceptin, show differential sensitivity between HER2-positive multi-spheroids (SKOV3 and BT474).https://www.essenbioscience.com/en/