Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 19

Real-Time Analysis of Advanced Cell Models | ADVANCED IN VITRO MODELING

The BT-474 multi-spheroids appeared more sensitive to Herceptin cytotoxicity with
the highest test concentration of Herceptin (1 µg/ml-1) producing approximately
80% inhibition compared with approximately 50% in SKOV3 multi-spheroids.

the impact of stromal cells on tumor multi-spheroid resistance to chemotherapeutic
agents. Brightfield in combination with fluorescence imaging permits visualization
and quantification of immune cell-mediated toxicity within tumor multi-spheroids.

Conclusion
The IncuCyte S3 Live-Cell Analysis System enables the analysis of spheroid biology in
real-time. Brightfield, in combination with phase-contrast imaging allows for labelfree assessment of changes in multi-spheroid morphology and size. With no need for
selection of a predefined end-point, consistent segmentation and quantification of
brightfield images enables kinetic assessment of cell-dependent growth profiles and

More advanced 3D models that incorporate an ECM and additional cell types have
the potential to provide more relevant translational models for the study of the
tumor micro-environment on tumor biology. 	
n

Figure 5.

Figure 5. Perform ADCC immune cell killing of HER2 positive multi-spheroids. PBMCs were cocultured with tumor multi-spheroids either stably expressing nuclear restricted RFP (SKOV3NR, MCF7-NR) or cytoplasmically restricted GFP (BT-474-CyG). Images show effect of Herceptin
on spheroid proliferation in presence and absence of PBMCs. IncuCyte S3 brightfield object

19

| January, 2019

outline mask shown in yellow. Death quantified in real time as a loss of fluorescence intensity
within the spheroid BF object. Concentration response curves to Herceptin, show differential
sensitivity between HER2-positive multi-spheroids (SKOV3 and BT474).


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Gain Critical Insights from Advanced Cell Models with Real-Time Analysis

Table of Contents for the Digital Edition of Gain Critical Insights from Advanced Cell Models with Real-Time Analysis

Contents
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 1
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - Contents
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 3
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 4
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 5
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 6
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 7
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 8
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 9
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 10
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 11
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 12
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 13
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 14
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 15
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 16
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 17
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 18
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 19
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 20
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 21
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 22
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 23
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 24
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 25
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 26
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 27
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 28
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 29
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 30
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 31
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 32
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 33
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 34
Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 35
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