Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 29

Real-Time Analysis of Advanced Cell Models | WHITE PAPER

Overall, the new approach outlined here provides a further dimension
to live-cell analysis, where researchers can now probe questions at the
cell-by-cell level and drill down into the characteristics and behaviors of
subsets of cells. Increasingly, as live-cell analysis is applied to more complex and advanced cell systems with intrinsic heterogeneity, this analysis
solution set will yield the additional biological insight promised by these
models.
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| January, 2019

A. Phase and Red

C. Green + Mask

B. Phase + Mask

D. Backgating Mask

Figure 9: CD8 positive T cell enrichment in immune/tumor cell co-cultures. (A-C) Identification of
hPBMCs (phase, yellow mask) and CD8+ (green - FabFluor 488 anti-CD8) subset in co-cultures with
A549-Cytolight Red labeled tumor cells. (D) Classified segmentation mask: purple = CD8+, cyan= CD8.
(E) Activation with anti-CD3/IL2 induced a marked proliferative effect in the total population and
enrichment of the CD8 subset (35% at t=0 vs 60% @ 72 h).

https://www.essenbioscience.com/en/ https://doi.org/10.1371/journal.pone.0102678 https://doi.org/10.1371/journal.pone.0102678 https://doi.org/10.1073/pnas.171534511

Gain Critical Insights from Advanced Cell Models with Real-Time Analysis

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