Gain Critical Insights from Advanced Cell Models with Real-Time Analysis - 5

Real-Time Analysis of Advanced Cell Models | A CELLULAR JOURNEY BEGINS

This is especially important for imaging cells that do not adhere well, such as immune cells, which go into suspension when the culture vessel is moved, and eventually resettle with gravity but not necessarily in their original location. The IncuCyte
optics obviate any culture vessel movement, and the camera registration allows
observation of individual cells over time.
A single IncuCyte accommodates up to six tissue culture flasks, dishes, 96-well or
384-well plates, and can generate in excess of 2000 parallel time-lapse movies, in
real time for long-term, live-cell phenotypic assays. Data acquisition and analysis
are automated.
The flexible live-cell analysis system can be used for a myriad of applications in immunology, immuno-oncology, cancer, and neuroscience research, says Dr. Trezise.
"We take an application-based approach by focusing on a specific scientific question and then develop and integrate all the building blocks, including protocols,
reagents, and software modules, needed to answer that question. A common misconception is that reagents used for end-point measurement are also applicable to
live-cell imaging. To illustrate the differences, immunocytochemistry requires that
the cells are fixed in an irreversible way; these reagents are not translatable to livecell immunocytochemistry," adds Dr. Trezise.
Automation Limits Variables
Many assays can be time consuming and challenging when performed manually.
Human intervention influences the physiology of manually-generated data. Automation is preferable to eliminate the human variable.
For example, cell migration and invasion play a role in many normal and pathological processes, including immune responses, embryonic development, angiogenesis, regeneration, tumor metastasis, and wound healing. Migration scratch assays
permit velocity measurements of wound closure.
According to bench scientists, when migration scratch assays are performed manually, cells are cultured in a plate, and a cut or scratch made, well by well with a pipette
tip, and then images are acquired at defined time points using a conventional mi5

| January, 2019

The flexible
live-cell
analysis
system can
be used for
a myriad of
applications in
immunology,
immunooncology,
cancer, and
neuroscience
research, says
Dr. Trezise.

https://www.essenbioscience.com/en/

Gain Critical Insights from Advanced Cell Models with Real-Time Analysis

Table of Contents for the Digital Edition of Gain Critical Insights from Advanced Cell Models with Real-Time Analysis