Multiplexing Phenotype and Function for More Biologically Relevant Insights - 11

Using the Intellicyt® iQue Screener PLUS to Profile
Antibody Dependent Cell Cytotoxicity
Caroline Weldon*, Catriona Thomson+,
Ben Tyrrell+, John ORourke*

D

uring the antibody discovery workflow,
functional studies are needed to screen
for desired antibody characteristics.
Antibody mutational screening and other
processes to increase antibody potency
or functionality require multiple rounds of
monitoring. Therapeutic antibodies often
mediate direct killing of cancer cells through
antibody dependent cell-mediated cytotoxicity (ADCC). Traditional ADCC assays use a
homogenous live/dead readout, which greatly
limits the contextual and correlative value of
the screening data. To overcome this limita-

*Essen Bioscience, Inc., formerly IntelliCyt Corporation,
+Sartorius Stedim BioOutsource, Glasgow Scotland

Figure 1: ADCC Assay. VL1 encoded CD20 positive
cells were cultured with various dilutions of
Truxima and with PBMCs (E:T of 0, 10 or 20).
Target cell viability was measured by BL1 median
fluorescence intensity, live cell counts and dead
(BL1 positive) target cells as a percentage of total
target cells. In all parameters, cell death rose
with increasing antibody concentration in the
presence of effector cells. Cell death occurred at
the high antibody concentration in the absence
of effector cells, consistent with published in vitro
studies using CD20 antibodies.

11

| January, 2019



Multiplexing Phenotype and Function for More Biologically Relevant Insights

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Multiplexing Phenotype and Function for More Biologically Relevant Insights - Contents
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