Multiplexing Phenotype and Function for More Biologically Relevant Insights - 12

More Biologically Relevant Insights | CELL CYTOTOXICITY

tion, a high throughput, multiplex screening assay profiling ADCC was developed
and analyzed using the Intellicyt® iQue Screener PLUS and integrated ForeCyt®
software. This screening assay can discriminate between effector and different target
cell populations in the same sample, and performed in 96 or 384 well plates. The
Panorama feature in ForeCyt allows for easy data visualization, even in large, multiplate screening campaigns.

Figure 2: ADCC Antibody Specificity. VL1 encoded
CD20 positive and CD20 negative cells were cultured
in each well. The ADCC assay was performed and
data was analyzed as in Figure1.Concentration
dependent cell death was observed in the CD20
positive cells, but no loss in cell viability was seen in
the CD20 negative cell lines

12

| January, 2019

Cells and Reagents: The CD20 positive human B-cell lines Ramos and Raji (ATCC,
Manassas VA) were used for the studies. The CD20 negative HL-60 cell line was
used in multiplex assays. Cryopreserved PBMCs were purchased from Astarte
Biologics (Bothell WA) and cultured for 20-24 hours prior to the assay. Truxima was
a generous gift from BioOutsource (Glasgow Scotland). Anti-hCD20 IgG1, IgG2 and
IgG mutants were purchased from InvivoGen (San Diego CA).


https://www.intellicyt.com/

Multiplexing Phenotype and Function for More Biologically Relevant Insights

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Multiplexing Phenotype and Function for More Biologically Relevant Insights - Contents
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