Multiplexing Phenotype and Function for More Biologically Relevant Insights - 23

More Biologically Relevant Insights | TUTORIAL

containing cells and supernatant, were transferred to an assay plate and analyzed
using the T Cell Activation Cell and Cytokine Profiling Kit workflow.

activator compound. T-cell activators do not all use the same pathways; the pathway
influences the strength of response, and the levels and types of secreted cytokines.

Data was acquired on the Intellicyt® iQue Screener PLUS and analyzed using the
integrated ForeCyt® software.

Conclusion
To perform an effective screening campaign, the screening technologies and
reagents utilized must not only have the speed and sensitivity to support highthroughput but also be cost effective and able to provide meaningful data.

The data in Figures 3a and 3b show dose and temporal responses in the percentage
of activated T cells and the amount of secreted IFNγ among the different activation
treatments. Unique patterns of T-cell activation markers were observed with each
Figure 2.

23

| January, 2019

Continues on page 25


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Multiplexing Phenotype and Function for More Biologically Relevant Insights

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