Multiplexing Phenotype and Function for More Biologically Relevant Insights - 7

More Biologically Relevant Insights | MORE DATA... MORE INSIGHT

consumption to a level that increased the screener's superiority compared with the
other immunoassays, notes Dr. ORourke
Automating Processes Start to Finish
In a 2018 publication Takeda Pharmaceuticals described a high-throughput flow
cytometry (HTFC) workflow, using the Intellicyt® iQue Screener PLUS and a six-sided
modular HighRes MicroStar robotic system, to take advantage of the screener's
acquisition speed, sample miniaturization, and plate-based data management. The
automated workflow incorporated all of the tedious sample preparation, increased
throughput, and provided precise control for the timing of each step to ensure more
consistent and robust results, plate-to-plate and run-to-run.
The results demonstrated that multiplexing mouse IgG capture beads and cells in
a primary hybridoma screen could potentially add more information during the
decision-making process. The authors indicated that the application of cytokine
capture beads can be used to monitor multiple cytokine levels and cell activation
status simultaneously, and that the HTFC was capable of providing high-content

Figure 2. Kite Pharma assay
schematic and flow panel. (A)
Schematic of co-culture cytotoxicity
assay depicting antigen-positive
and-negative adherent tumor cells
(purple and blue) and T cells (green).
(B) Flow cytometry panel, listing
markers measured and fluorescent
channels used. (C) Equation used
to calculate the percentage of
chimeric antigen receptor (CAR)-T
cell-dependent cytotoxicity in the
co-culture assay.3

7

| January, 2019

information during an antibody generation campaign.2
The system's wide dynamic range allows multiplexed cell and bead assays in the
same well for full cell phenotyping and functional analysis. A suite of available
reagents evaluate phenotypic changes, cell health markers, such as viability and
proliferation, and also quantitate in a bead-based ELISA the amount of secreted
cytokines or IgG. Instead of using multiple assays on different instrumentation
everything can be done in a single well, says Dr. ORourke.
Combining Multiplex Capabilities with Ease of Use
LifeArc, a not-for-profit organization, pioneers new ways to turn great science into
greater patient impact by collaborating with academic researchers and other groups
to translate basic research into lead-stage therapeutic assets.
"Our flow cytometry capabilities prior to the Intellicyt iQue Screener were plate
based so somewhat high throughput in nature, but the machine setup and read
times were frustratingly lengthy, while the flow cell was prone to blocking. Now

https://www.intellicyt.com/

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