Jax Labs eBook - 20

IMPACTFUL SOLUTIONS CREATE INNOVATIVE SCIENCE

grandchildren (Figure 2), enough to last
for 25-30 years (Reardon, 2017), and
which are periodically used to replenish
pedigreed foundation breeding colonies
(Wiles and Taft, 2010). The Jackson
Laboratory's unique, patented Genetic
Stability Program (GSP) effectively limits
cumulative genetic drift, including that
caused by copy number variation (CNV),
by rebuilding our foundation stocks from
cryopreserved, pedigreed embryos every
five generations. This process introduces
a controlled bottleneck that minimizes
the accumulation of genetic change.
Therefore, B6J mice obtained from JAX
will be a maximum of seven generations
removed from Eve (Reference sequence
GRCm39).
These findings point to the genetic
distance between the original B6J mice
sequenced in 2002 compared to Eve's
within JAX. In addition, they illustrate
the risks of substrain divergence when
researchers or vendors maintain colonies
using "traditional" inbreeding methods
without programs to minimize the
20 | GENengnews.com

accumulation of spontaneous mutations
through generations whose sequence will
not correspond tightly to the Reference
Genome data.
Learn more about JAX mice and
services, including the patented GSP, at
the Why JAX Mice page. nn

REFERENCES
Mouse Genome Sequencing Consortium, Waterston RH,
Lindblad-Toh K, Birney E, Rogers J, Abril JF, Agarwal
P, et al. Initial sequencing and comparative analysis of
the mouse genome. Nature. 2002 Dec 5;420(6915):520-62.
PMID: 12466850
Genome Reference Consortium Mouse Build 38 patch release
6 (GRCm38.p6) https://www.ncbi.nlm.nih.gov/assembly/
GCF_000001635.26
McGarvey KM, Goldfarb T, Cox E, et al. Mouse genome
annotation by the RefSeq project. Mamm Genome. 2015;26(910):379-390. doi:10.1007/s00335-015-9585-8
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602073/

RECOMMEND RESOURCES
Webinar: Protect Your Research Know Your B6 Mouse
Webinar: Achieving Reproducible Mouse Studies
Strategies to Maximize the Reproducibility of Mouse Research
Remember only YOU can prevent genetic drift

Reardon S. 2017. Lab mice's ancestral 'Eve' gets her genome
sequenced. Nature. 2017 Nov 13;551(7680):281. doi: 10.1038/
nature.2017.22974. PMID: 29144484.
Sarsani et al 2019. The Genome of C57BL/6J "Eve", the
Mother of the Laboratory Mouse Genome Reference Strain.
G3 (Bethesda). 2019 Jun 5;9(6):1795-1805. doi: 10.1534/
g3.119.400071. PMID: 30996023
Genomic version LXEJ02000000:
Mus musculus strain C57BL/6J isolate Eve, whole genome
shotgun sequencing project consists of sequences
LXEJ02000001-LXEJ02012661
GenBank: LXEJ00000000.2
https://www.ncbi.nlm.nih.gov/nuccore/LXEJ02000000
B6Eve assembly along with annotation and an assembly hub:
ftp://ftp.jax.org/b6eve
Visualization of the assembly: https://genome.ucsc.edu →
MyData →Track Hubs → My Hubs with the following
URL: ftp://ftp.jax.org/b6eve/assemblyhub/hub.txt
Wiles and Taft 2010. The sophisticated mouse: protecting a
precious reagent. Methods Mol Biol. 2010;602:23-36. doi:
10.1007/978-1-60761-058-8_2. PMID: 20012390

Jax Labs eBook

Table of Contents for the Digital Edition of Jax Labs eBook

Contents
Jax Labs eBook - 1
Jax Labs eBook - 2
Jax Labs eBook - 3
Jax Labs eBook - Contents
Jax Labs eBook - 5
Jax Labs eBook - 6
Jax Labs eBook - 7
Jax Labs eBook - 8
Jax Labs eBook - 9
Jax Labs eBook - 10
Jax Labs eBook - 11
Jax Labs eBook - 12
Jax Labs eBook - 13
Jax Labs eBook - 14
Jax Labs eBook - 15
Jax Labs eBook - 16
Jax Labs eBook - 17
Jax Labs eBook - 18
Jax Labs eBook - 19
Jax Labs eBook - 20
Jax Labs eBook - 21
Jax Labs eBook - 22
Jax Labs eBook - 23
Jax Labs eBook - 24
Jax Labs eBook - 25
Jax Labs eBook - 26
Jax Labs eBook - 27
Jax Labs eBook - 28
Jax Labs eBook - 29
Jax Labs eBook - 30
Jax Labs eBook - 31
Jax Labs eBook - 32
Jax Labs eBook - 33
Jax Labs eBook - 34
Jax Labs eBook - 35
Jax Labs eBook - 36
https://www.nxtbookmedia.com