Jax Labs eBook - 32

IMPACTFUL SOLUTIONS CREATE INNOVATIVE SCIENCE | QUICK FACTS

WHY SHOULD I MEASURE THE HALF-LIFE OF MY THERAPEUTIC?
Antibody half-life can be affected by pH, by modifications made to variable regions,
by choice of linkers, or by the type of payload attached, as in the case of antibodydrug conjugates. Because of this, you may need to test the half-life of several variant
molecules before moving forward with a particular candidate.

A D D I T I O N A L CO N T E N T

Unravel the drug discovery
path with JAX G O THER E

HOW DO I MONITOR HALF-LIFE?
The kinetics (Kd) of human FcRn interactions can be quantified quickly and relatively
inexpensively using in vitro methods like cellular binding assays and Surface Plasmon
Resonance-based approaches. Eventually, clinically-relevant pharmacokinetic values
such as half-life will need to be characterized.
Typically, in vivo PK measurements are done by injecting the candidate molecule
intravenously into an animal model and measuring levels of the molecule in the serum
over several hours and days. (Figure 3).
Figure 3. Typical in vitro and in vivo methods to
measure antibody half-life. Adapted from
www.sprpages.nl and Kamath et al., 2016.

32 | GENengnews.com


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Jax Labs eBook

Table of Contents for the Digital Edition of Jax Labs eBook

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