Optimize, Adapt and Overcome - 10

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Single-Cell Printing for Establishing Monoclonality

identify a single cell in a label-free image, it is also
much easier to confirm the absence of a second cell
in the sample, further improving the assurance of
monoclonality.
The improvements to efficiency, viability, and
assurance of clonality shown with the CloneSelect
Single-Cell Printer compared with traditional methods
will have immediate and obvious impact on time
and costs associated with cell-line development
work flows. Expected clonal outgrowth using the
SCP (combining efficiency and viability) is, at minimum, 60%; whereas flow cytometry and limiting
dilution typically do not exceed 25%. Thus, fewer
plates, less media, and less time are required to perform the same work using the SCP approach. Less
obvious, but just as impactful, are the advantages
SCP brings to downstream processes as well.
Due to the inefficiencies associated with current
techniques, most researchers have to cherry-pick
their clones prior to screening for titer. This

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process can be labor-intensive and error-prone if
done manually, or alternatively, it may require an
expensive liquid handler. With the high efficiencies
achieved by the SCP, cherry-picking may no longer
be required, simplifying the liquid-handling process,
whether it is done manually or through automation.
Taken together, these benefits allow for researchers
to screen more clones, in less time, at reduced cost,
and with higher confidence that a given cell line is
monoclonal. n
Reference
1. A. Mayer-Bartschmid, "Evaluation of New Single Cell
Deposition Technologies to Ensure Monoclonality,"
Presentation at the meeting of Cell Line Development
and Engineering Amsterdam, NL (April, 2017).

Sarmad Al-Bassam, Ph.D., serves as
applications scientist and Steve Wiltgen, Ph.D.
(steve.wiltgen@moldev.com), is product
manager at Molecular Devices.
Website: www.moleculardevices.com.

http://www.moleculardevices.com http://www.GENengnews.com

Optimize, Adapt and Overcome

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