Optimize, Adapt and Overcome - 17

Figure 3. Finding a suitable concentration of calcein AM
to optimize detection at the single cell level.

17

| GENengnews.com

(A) High-density wells containing cells stained with 5 μM CAM shows
consistent detection of cell signal. (B) High-density wells containing
cells stained with 1 μM CAM shows reliable detection of cell signal. (C)
High-density wells containing cells stained with 0.5 uM CAM shows that
only a minority of cells can be detected based on the low fluorescence
intensity signal. (D-F) Fluorescence scan of plate using CSI from limiting
dilution using 5 μM, 1 μM, and 0.5 μM CAM cell staining. (G) Cell per well
count tally for the 5, 1, 0.5 μM CAM cell staining and comparison with
theoretical values from limiting dilution at 0.5 cells per well. Comparison
of single cell counts (dashed line). (H) Percent divergence of observed and
predicted single cell data. >90% for 0.5 μM CAM compared with <5% for
1 and 5 μM CAM.



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Optimize, Adapt and Overcome

Table of Contents for the Digital Edition of Optimize, Adapt and Overcome

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Optimize, Adapt and Overcome - 1
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