Optimize, Adapt and Overcome - 19

Figure 4. Measuring the effect of different calcein AM
concentrations on viability.
(A) Day 10 clonal outgrowth of representative plates from
label-free, 0.5 μM, 1 μM, and 5 μM calcein AM stained cells
before limiting dilution. (B) Quantification of raw outgrowth of
single colony-containing wells. (C) Representative single colonycontaining wells at day 6. (D) Quantification of colony size (surface
area) shows that colonies derived from 5 μM CAM cell staining
are ~40% smaller in size compared with label-free and lower CAM
cell staining conditions. (E) Quantification of single colony wells
derived from single cells shows consistent data to raw outgrowth
percentages outlined in (B).
19

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Optimize, Adapt and Overcome

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