Optimize, Adapt and Overcome - 21

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Confident Assurance of Clonality Using Calcein AM with Minimal Effect on Viability

the appropriate concentration of dye requires optimization across a number of parameters (cell type,
imaging system, media, etc.). The imaging of microplates in fluorescence is inherently slower than
in brightfield and must be considered when high
throughput workflows are desired. Determining
whether brightfield or fluorescence imaging is most
appropriate will then depend upon the individual
workflow. Table 1 summarizes some of the key differences between both workflows to help guide the
choice between the two workflows. The CloneSelect
Imager with fluorescence is able to meet the needs
for either workflow though as shown in this application note, alleviating the need to make a choice
between instruments when choosing a particular
workflow. n

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Table 1. Comparing the WL and FL workflows to assessing monoclonality.

Molecular Devices
Phone: +1.800.635.5577
Web: www.moleculardevices.com
Email: info@moldev.com
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Optimize, Adapt and Overcome

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