Optimize, Adapt and Overcome - 24

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Enhanced Development of Virus-Specific Hybridomas Using ClonePix and CloneSelect Imager Technologies

Figure 1. Visualization of IgG secretion in parental hybridoma
cell line suggests only a small subset (~7%) is producing ds-DNA
virus-specific antibody.
Hybridoma cells were plated at 200 cells/mL in 6-well plates in CloneMedia
Semi-Solid Media (for hybridomas/myelomas). Fluorescently conjugated
CloneDetect Agent was added to enable in situ detection of secreted IgG.
Colonies were imaged on a ClonePix System in white light at 150 ms (A) and
fluorescence at 500 ms (B). Variability of FITC signal as quantitated by Interior
Mean Intensity and Exterior Mean Intensity of the parent line indicate an
unstable, non-monoclonal hybridoma cell line.
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Figure 2. ClonePix Technology Principle. A: Concept overview
of antibody detection from individual hybridoma clones grown
suspended in a methylcellulose matrix. B: Whitelight image of
precipitated antibody sequestered around a growing hybridoma
clone. C: ClonePix System detection and selection of acceptable
target clones. D: ClonePix System automated picking and transfer
of isolated target clones to destination plates.

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Optimize, Adapt and Overcome

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