Optimize, Adapt and Overcome - 28

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Enhanced Development of Virus-Specific Hybridomas Using ClonePix and CloneSelect Imager Technologies

Figure 5. Sub-cloning of parental hybridoma by ClonePix results in uniform
IgG secretion at higher yields. A subset data for one sub-clone is shown.
A: A significant improvement in % FITC-positive colonies as a result of sub-cloning
is observed as compared to the parental clone (Figure 1 A, B). IgG secretion was
detected at seven days growth by 100 U/mL of CloneDetect Reagent. B: Scatter plot
shows a linear correlation between Exterior and Interior Mean Intensity; the slope is
shifted towards the Y-axis indicating greater uniformity, while more clones in upper
quadrants signify the presence of higher FITC positive clones.

Figure 6. ds-DNA virus IgG clone production capacity.
Sub-cloning by ClonePix System resulted in a dramatic increase
in cell line titer.

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Optimize, Adapt and Overcome

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