Optimize, Adapt and Overcome - 29

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Enhanced Development of Virus-Specific Hybridomas Using ClonePix and CloneSelect Imager Technologies

Conclusion

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ClonePix Technology improves both the timeline and quality
of screened hybridoma sub-clones by decreasing manual
manipulation, while CloneMatrix Media and CloneDetect
Reagent allow improved growth and high sensitivity detection
of secreting clones. Data from the ClonePix System suggests
that the poor production capacity of the parent clone was due
to a small subpopulation of antibody producing cells that were
being overtaken by faster growing,
non-producing cells.

Molecular Devices
Phone: +1.800.635.5577
Web: www.moleculardevices.com
Email: info@moldev.com

Used in conjunction with CloneSelect Imager, the ClonePix
System had proven to be a powerful tool in identifying two
high-yield, high-affinity hybridoma sub-clones capable of
significantly higher antibody production capacity (17-25 mg/L)
as compared to the previous production attempts of the parental
line hybridomas (0.5-1 mg/L). Thus, this technology effectively
contributes to the development of a novel therapeutic antibody
to detect specific ds-DNA virus. n

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Optimize, Adapt and Overcome

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