Optimize, Adapt and Overcome - 32

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Screening and Selection of GPCR-Expressing Cell Lines

The ClonePix 2 system (Figure 1) utilized brightfield to identify the colonies' morphology and
location, and fluorescence to identify the M1
expression. Positively expressing CHO-M1 cells
produced a range of fluorescent signals with
both the direct-labeled and dual-antibody
approach. The parental line CHO-K1 did not
produce a fluorescent signal (Figure 2). Clones
were ranked by fluorescent intensity and binned
into groups based upon intensity: high, medium,
low, and no signal.

Figure 1. ClonePix 2 System reveals diverse levels of fluorescent intensity with the CHO-M1
cell line, showing it can distinguish between various levels of expression of GPCR M1 protein.
Colonies recognized by the software are outlined in color under the brightfield channel.
Fluorescence intensity is calculated based upon physical location of colonies.

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Optimize, Adapt and Overcome

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