Optimize, Adapt and Overcome - 37

Optimize, Adapt, and Overcome: Tips from the Cell Line
Development Front * How to Improve Cell-Line Productivity

E X P E R T PA N E L

and finding highly expressing clones through optimizing
selection systems, expression vectors, and screening.
Lately, development of chemically defined media and feeds
combined with accessibility to high-throughput analytical
tools allow better understanding of nutrient influence on
the quality of expressed protein. I believe that including
feeds and protein quality testing early in clone screening is
beneficial for selection of clones with higher protein quality.
Dr. Racher
Both contribute to product quality and productivity; it depends upon your objective. Current process productivities
are a combination of an effective protein expression system,
a good host cell line, selection of a suitable product variant,
appropriate media feeds, and process operating conditions.
The importance of clone selection depends on whether
you need "good enough" productivity or require a highproducing outlier to meet projected demand; this is more
important if a specific quality attribute is desired. A selected
clone gets you close to the target. Then you can use
modifications to media feeds and process to optimize
to the specific target attribute.

| GENengnews.com

Donato
Tedesco, Ph.D.

Lead Research Scientist
Cellecta

Andy
Racher, Ph.D.

Associate Director
Future Technologies
Pharma Biotech,
Lonza

Renata
Gregory
Pankiewicz, Ph.D. Alberts, Ph.D.
Laboratory Manager
Fusion Antibodies

Erika
Holroyd

Product Manager
CHOZN® Program
MilliporeSigma

Igor
Fisch, Ph.D.

Global Subject Matter
Expert, Lonza Pharma
Bioscience Solutions

CEO and Chairman
Selexis

Mark
Powers, Ph.D.

Senior Director
Cell Biology R&D
Thermo Fisher Scientific

http://www.GENengnews.com

Optimize, Adapt and Overcome

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