Optimize, Adapt and Overcome - 5

Single-Cell Printing
for Establishing
Monoclonality
Utilizing Microfluidics and Imaging to
Improve the Cell-Line Development Workflow
Sarmad Al-Bassam, Ph.D., and
Steve Wiltgen, Ph.D.

T

he increasing adoption of large protein
biologics such as monoclonal antibodies
(mAbs) for the treatment of disease has heralded
unprecedented growth in the R&D of novel
therapeutics and biosimilars, bringing unique
challenges to the biopharmaceutical industry.
In a typical workflow to cultivate cell lines, the
gene that encodes the recombinant protein of
5

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interest is transfected into a cell line of choice
(typically CHO), where copies of the gene are
incorporated at multiple, random sites within the
genome. Establishment and optimization of a cell
line (referred to as cell-line development) entails
finding the rare clones where random integration
results in high, stable production of the therapeutic
with desirable consistency and quality attributes.
In addition, this optimization must be accomplished
under the scrutiny of regulatory agencies that require
thorough demonstration of product safety and

ADDITIONAL CONTENT

Webinar Replay
A Microfluidics-Based Single Cell Isolation
Workflow Optimized for Efficiency, Viability,
and Assurance of Monoclonality
Watch Webinar

https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/microfluidics-based-single-cell-isolation-workflow-optimized-for-efficiency-viability-and-assurance-of-monoclonality?utm_source=gen&utm_medium=ebook http://www.GENengnews.com

Optimize, Adapt and Overcome

Table of Contents for the Digital Edition of Optimize, Adapt and Overcome