Optimize, Adapt and Overcome - 9

Optimize, Adapt, and Overcome: Tips from the Cell Line Development Front * Single-Cell Printing for Establishing Monoclonality

The use of imaging on the SCP has several additional benefits beyond improving efficiency and
viability. Images of single cells captured on the SCP
provide high assurance of monoclonality through
direct visual evidence (Figure 3). This has been
validated by capturing images of the same cells
post-printing with the CloneSelect Imager (an
automated, label-free imager). A high degree of
correlation (>98%) was observed between the
single-cell images captured on both systems.
The CloneSelect Imager (CSI) adds significant value
to this workflow by providing a second, independent measure of clonality, thereby substantially
improving the probability of clonality. In addition,
the CSI can also characterize the growth pattern
and morphology of candidate clones, further
complementing the cell-line development workflow downstream of single-cell printing. An additional benefit to imaging on the SCP is that the
nozzle is significantly smaller than the vast space of
the microplate surface, allowing for quick confirmation of monoclonality. Not only is it much easier to

| GENengnews.com

Figure 3. A sequence of five images at the nozzle portion of the cartridge provides evidence
for single-cell deposition. The sequence illustrates the path of a single cell prior to and
following ejection from the nozzle tip.
Images 1-3 show the cell approaching the nozzle.
Image 4 shows the detection of a single cell (inner circle) and the absence of any cells
in the vicinity (outer circle).
Image 5 shows the nozzle after droplet ejection to provide evidence that the single
cell was expelled from the nozzle.

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Optimize, Adapt and Overcome

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