ProteinSimple eBook - 10

The
Mastering Charge Heterogeneity
Analysis of Therapeutic Proteins
ELISA-free

10

| GENengnews.com

provides high-resolution charge heterogeneity peak
proﬁles in 10 minutes. In this tutorial, we describe
a simple and easy approach for icIEF method
development on the iCE3 along with tips to
improve method robustness.

Additional Content
Application Notes:

Computer-Aided Assay
Imaged cIEF Principle
Development for Charge
Meet Ella. She's simple. Simply irresistible. With moves like J
Heterogeneity Analysis by iCE.
Signiﬁcantly different from traditional cIEF,
bass and treble,
the iCE3 performs capillary IEF with whole- she leaves your old-fashioned ELISA doing th
column detection, which eliminates the need what? The Simple Plex is here.
Turn down for for
a lengthy mobilization step-this both increases
Learn more!

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nalyzing charge variants of biopharmaceuticals
is a critical component of product development
and quality control. Charge variants commonly
occur as a result of both chemical and posttranslational modiﬁcations including
deamidation, oxidation, glycosylation, and
glycation. These changes can affect biological
activity, patient safety, and drug stability.
Charge variants have traditionally been
monitored by ion exchange chromatography
(IEC), but newer CE-based techniques, such as
imaged capillary isoelectric focusing (icIEF), offer
the advantages of generic methods for multiple
products and faster analysis times. Analysis of
biopharmaceuticals using icIEF on the iCE3 system

sample throughput and reduces assay complexity.
Two electrolytic tanks at each end of the cartridge
are ﬁlled with acid (anolyte) and base (catholyte).
Prepared samples for icIEF contain a mixture of

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