ProteinSimple eBook - 8

Simplifying Protein Analysis * Westerns and Total Protein Analysis

Expanding Simple Western Applications
We at ProteinSimple like challenges and are
always on the lookout for problem areas in
protein research that need a little (or a lot of)
simplifying, like total protein analysis. We
found a way to simplify that too with the
Simple Western Total Protein Assay. It lets

Figure 3. An entire cell signaling pathway analyzed in one
run by Sally Sue.

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you detect all proteins in your sample without
running a single gel, and does it with Simple
Western simplicity. Without the need for
antibodies, a biotin reagent binds to the
proteins and is detected by chemiluminescence
using streptavidin-HRP (Figure 4).

Figure 4. Total protein and immunoassay detection of decreasing concentrations of DNAK in
HeLa lysate (15, 7.5 and 3.75 μg/mL in the Total Protein Assay, 0.015, 0.0075 and 0.00375 μg/mL
in the immunoassay) performed in Wes.

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