Sartorius eBook - 20

National Institute of Allergy and Infectious Diseases-Rocky Mountain Laboratories, NIH

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

This scanning electron microscope image shows SARS-CoV-2 (orange)-also known as 2019-nCoV, the virus that
causes COVID-19-isolated from a patient in the U.S., emerging from the surface of cells (green) cultured in the lab.

intestines. The scientists also found that the virus

virus in the stool of patients free of respiratory

provoked the activity of genes involved with

symptoms. Special attention may be needed for

antiviral responses. Notably, the rates of infection

those patients with gastrointestinal symptoms.

were similar across the organoid models,

More extensive testing using not only nose

indicating that even low quantities of ACE2 may

and throat swabs, but also rectal swabs or stool

be enough for the virus to infect epithelial cells.

samples may thus be needed.

"The observations made in this study provide

In the meantime, the researchers are

definite proof that SARS-CoV-2 can multiply

continuing their collaboration to learn more

in cells of the gastrointestinal tract," said Bart

about COVID-19. They are studying the

Haagmans, PhD, a corresponding author of

differences between infections in the lung and

the current study and a researcher at Erasmus

the intestine by comparing lung and intestinal

University Medical Center. "However, we don't

organoids infected with SARS-CoV-2.

yet know whether SARS-CoV-2, present in the
intestines of COVID-19 patients, plays a significant
role in transmission. Our findings indicate that we
should look into this possibility more closely."
The current study is in line with other recent
studies that identified gastrointestinal symptoms
in a large fraction of COVID-19 patients and
20 |

GENengnews.com

ADDITIONAL CONTENT
Protocol: This protocol provides an
overview for culturing, monitoring and

Protocol

measuring organoid growth, counts
and morphology, using an Incucyte®
Live-Cell Analysis System and Incucyte®
Organoid Analysis Software.

Incucyte

®

Organ

oid Cul
For the
ture QC
Quantifi
cation of
Matrige
Organo
l Dome
id Grow
s
th
®

This proto
col prov
ides
and mea
suring orga an overview
for cultu
of Brigh
ring, mon
tfield imag noid growth,
counts
itoring
Incucyte ®
es in Matr
Live-Cel
igel ® dom and morpholo
Organoid
gy
l Analysis
es utiliz
ing the
Analysis
System
Software.
and Incu
cyte ®

General

Guideline

Require
24-well

in

d Mater

ials

or 48-w
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om
Follow
Cat. No.
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Organoid of interest
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to blow
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allow the the Incucyte Live- media changes,
Coolsink
StemCell
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Technolo
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37° C for
No. 7093
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Pancreati
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Hep
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IntestiCu atic Organoid
s Cat. No.
s Cat. No.
lt™ OGM
70933;
70932
Cat. No.
Cat. No.
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ult™ OGM
Cat. No.
06030

s


https://www.essenbioscience.com/media/uploads/files/Organoid-Culture-QC-Protocol-en-8000-0733-A00-Incucyte.pdf http://www.GENengnews.com

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