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THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS
better-informed decision-making, for example,
1. Live Cell Monitoring Tools
the best time to manipulate a culture or perform
Provide Crucial Insights into the
an assay. Should unexpected cellular events
Quality of Cell Culture Assays
occur, they can be identified in real-time and
As innovative cellular models gather momentum
their effects interpreted, while captured data can
in the field of drug discovery, live-cell imaging
be retrospectively analyzed to further optimize
is proving invaluable for capturing cellular data
conditions for subsequent studies1. Live-cell
and dynamic insights. While assay readout may
monitoring can facilitate the success of all cell
be considered as the "Eureka moment" for many
culture assays by enhancing the development
researchers, it is important to emphasize that
and interpretation of relevant disease models
visualizing changes in cell culture over time is as
and complex assays, in concert with functional
crucial for assay QC as it is for the experimental
readouts.
output. The significance of this is highlighted in
This whitepaper illustrates the importance
a paper by Horbach et al., (2017)2, which showed
of live-cell monitoring for complex, recurrent
that more than 30,000 studies have reported
workflows and, with the ability to perform
research with misidentified cell lines. With the
multiparametric imaging techniques in a
scientific community focusing on increased rigor
non-perturbing manner, its relevance to the fields
and reproducibility for in vitro models3, live-cell
of immunology, oncology, and neuroscience are
monitoring provides a unique opportunity to
highlighted. Here, we describe different live-cell
ensure the quality and accuracy of assays.
monitoring tools and exemplify how they can
As a monitoring system, live-cell imaging
objectively provide reassurance and optimize
and analysis is a versatile technique that can be
data quality throughout the entire workflow,
used with or without labels to measure different
focusing on four advanced applications:
parameters depending on research needs. Label-
Live-cell monitoring tools for optimal QC
free approaches avoid the potentially damaging
of cell culture assays
effects of phototoxicity on cells associated with
Capturing complex cell manipulations,
conventional fluorescence microscopy4 and so
such as differentiation and activation
play a vital role in cell QC throughout cell culture
Measuring stem cell events, such as
workflows. For example, by detecting changes to
reprogramming and differentiation
cell coverage on the well surface, label-free live-cell
Analyzing the development of advanced
imaging can remotely monitor cells for weeks or
3D models
even months, providing quantitative confluence
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Sartorius eBook
Table of Contents for the Digital Edition of Sartorius eBook
Contents
Sartorius eBook - 1
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