Sartorius eBook - 24
THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS
and morphological information. Additionally,
timeframes. Therefore, in order to make
cell-by-cell analysis with label-free imaging can
informed decisions regarding cell husbandry,
measure individual cell dynamics and critical
it is essential to understand the timeline
morphological changes, which can characterize
of biological processes. Ultimately, robust
cell behavior in a heterogeneous population.
QC of cell cultures ensures that the models
Labeled approaches of cell imaging detect
are optimal from the outset, which in turn,
dynamic biological changes that cannot be
validates and improves the assay readout.
currently captured using label-free analysis. Live-
Integrating multiparametric real-time
cell immunocytochemistry (ICC), for instance,
dynamic monitoring and cell health measures
can detect changes in the expression of specific
into cell culture workflows can provide
surface proteins associated with defined stages
scientists with additional insight.
of cellular development, allowing important
cellular interactions and states to be detected
2. Live Cell Monitoring for
and tied to timepoints. By combining live-cell
Examining Cellular Events During
imaging and analysis with complementary
Differentiation and Activation
techniques such as ICC, further insights can
Owing to the complex and temporally dynamic
be found, for example, the measurement of
nature of neurological diseases, it is challenging
functional and morphological changes, which
to develop physiologically relevant models
allows researchers to create signatures for
in which to test novel treatments in this field.
culture manipulations.
In providing important insights about the
Furthermore, live-cell imaging can also utilize
pathogenesis of these disorders, in vitro models
technologies and techniques that are more
offer an appealing approach for the screening
closely tied to specific applications. For instance,
of potential pharmacological agents5. However,
live-cell imaging can use brightfield optics for
scientists investigating putative treatments for
the visualization of large and opaque structures,
neurological disease must ascertain that their cell
which is ideal for monitoring 3D structures and
culture is optimal, and because the phenotypic
spheroids, and different reagents can be used
assays may involve multiple manipulations,
to measure cell health, for example in apoptosis,
quality and standardization must be ensured at
cytotoxicity, and proliferation assays.
every stage.
Cutting-edge cell culture assays involve
multiple manipulations over extended
24 |
GENengnews.com
During neuronal development, neurons
extend processes (neurites) that differentiate
http://www.GENengnews.com
Sartorius eBook
Table of Contents for the Digital Edition of Sartorius eBook
Contents
Sartorius eBook - 1
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