Sartorius eBook - 26

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

events. For example, using continual monitoring
of neurite outgrowth and nuclear division as a

as well as modulation of signaling9.
By coupling CD71+ protein expression with

proxy for differentiation, scientists can ascertain

label-free phase-contrast, PBMC activation

the success of their differentiation assay and

can be associated with important timepoint-

objectively determine the developmental stage

dependent morphology changes, such as cell

of their neuronal cultures, thereby, allowing

size and shape (Figure 3). Live-cell ICC can also

them to progress to the next assaying stage. As

identify distinct cell subsets in heterogeneous

shown below, increased neurite outgrowth and

cell populations, while different cell types and

reduced cell division over time indicate neuronal

their interactions with targets can be monitored

differentiation (Figure 2A and C).

and characterized using multiple labels.

Cell cultures employed in innovative
immunology research are complex and

3. Dynamic Monitoring of

typically involve multiple manipulations, with

Reprogramming and Differentiation

QC underpinning the success of each stage.

During Stem Cell Culture Manipulation

Understanding the activity of immune cells,

Given their promising ability to recapitulate

such as peripheral blood mononuclear cells

patient-specific phenotypes, iPSC models are

(PBMCs), is essential for unraveling host defense

gaining traction in the fields of neuroscience,

mechanisms and disease pathogenesis, as

immunology, and oncology. Their growth in

well as performing toxicology testing for

popularity stems from their ability to generate

immunotherapies. Immune cell activation is a

humanized disease models in large quantities,

crucial manipulation stage for scientists studying

which makes them attractive for investigating

adaptive immune responses in vitro, and with

genetic mechanisms of cell repair, as well as for

PBMCs this can be stimulated by different

high-throughput drug screening applications10.

cytokines and antibodies.

Another area of great interest for iPSC use is in

Live-cell ICC tracks dynamic changes in protein

individualized treatment, whereby iPSC derived

localization as a function of time, instead of being

cells may help to evaluate drug effects on

limited to a single endpoint, as with conventional

individual genomic phenotypes.

ICC. Label-free imaging also captures changes

Unlike human embryonic stem cells that

to cell shape and structure, including cell

develop during embryogenesis, iPSCs are

eccentricity and elongation. Tracking these

artificially derived pluripotent cells. iPSCs are

morphological alterations can indicate cell health

essential research tools for disease modeling

26 |

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Sartorius eBook

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