Sartorius eBook - 27

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

Phase-contrast imaging and fluorescent surface labeling dynamically monitors PBMC activation
over time
A.

B.

Non-activated

Cell size
Average area
130 μm2

Activated

100
Non-activated

70

Activated

0

24

48
72
Time (h)

96

120

Cell shape
Average eccentricity
0.70

0.65

Activated, CD71

0.60

0.55

0

24

48
72
Time (h)

96

120

% CD71+ expression in subsets
CD71+
100 %

Figure 3: Visualization of PBMC activation using phase-contrast imaging and live-cell
ICC (anti-CD71 FabFluor-488). PBMCs (30K/well) were treated with anti-CD3 and IL-2,

Large

75
50

Small

or vehicle control, and labeled using anti-CD71 FabFluor-488. (A) Label-free phasecontrast imaging detects morphological differences between non-activated and

25

activated PBMCs; phase-contrast imaging coupled with live-cell ICC shows upregulated
CD71+ in activated cells. (B) Activation induces increased cell size, increased cell

0

0

eccentricity and upregulation of CD71+ in larger vs. smaller subsets of cells.

24

48
72
Time (h)

96

120

and developmental studies, showing huge

cell type, iPSCs can be created from an already

potential for functional screening assays10,11.

differentiated cell type.

By introducing stem cell-specific transcription
factors into a premature or mature somatic

Somatic cell reprogramming presents an
exciting opportunity to generate valuable iPSCs

GENengnews.com

| 27



http://www.GENengnews.com

Sartorius eBook

Table of Contents for the Digital Edition of Sartorius eBook

Contents
Sartorius eBook - 1
Sartorius eBook - 2
Sartorius eBook - 3
Sartorius eBook - Contents
Sartorius eBook - 5
Sartorius eBook - 6
Sartorius eBook - 7
Sartorius eBook - 8
Sartorius eBook - 9
Sartorius eBook - 10
Sartorius eBook - 11
Sartorius eBook - 12
Sartorius eBook - 13
Sartorius eBook - 14
Sartorius eBook - 15
Sartorius eBook - 16
Sartorius eBook - 17
Sartorius eBook - 18
Sartorius eBook - 19
Sartorius eBook - 20
Sartorius eBook - 21
Sartorius eBook - 22
Sartorius eBook - 23
Sartorius eBook - 24
Sartorius eBook - 25
Sartorius eBook - 26
Sartorius eBook - 27
Sartorius eBook - 28
Sartorius eBook - 29
Sartorius eBook - 30
Sartorius eBook - 31
Sartorius eBook - 32
Sartorius eBook - 33
Sartorius eBook - 34
Sartorius eBook - 35
Sartorius eBook - 36
Sartorius eBook - 37
Sartorius eBook - 38
Sartorius eBook - 39
Sartorius eBook - 40
Sartorius eBook - 41
Sartorius eBook - 42
Sartorius eBook - 43
Sartorius eBook - 44
Sartorius eBook - 45
Sartorius eBook - 46
https://www.nxtbookmedia.com