Sartorius eBook - 29

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

(Figure 4A). Continual and stable pluripotency

, such as brain. By utilizing live-cell monitoring

is imperative for using iPSCs in developmental

to acquire confluence measurements and

and regenerative medicine. Monitoring iPSC

observe morphological changes that are

clones as they emerge, and testing isolated

characteristic of neuronal maturation,

samples using ICC pluripotency markers, such

developing clones can be monitored as they

as SSEA4 and OCT4, provides reassurance for

emerge (Figure 5). The trajectory of cellular

scientists developing iPSCs (Figure 4B).

development and morphological changes can

A key advantage to iPSC models is that they

be highly variable with in vitro iPSC-derived

enable the development of humanized models

disease models and subtle, cumulative changes

of tissue that are typically inaccessible in situ

that may lead to unwanted phenotypic effects

Label-free and segmented imaging monitors iPSC-derived cell differentiation in real-time
A.

Oh

3d

6d

B.

Oh

3d

6d

Figure 5: Visualization of iPSC-derived neural progenitor cell differentiation into
cortical neurons using label-free phase-contrast imaging and segmentation masks.
Label-free phase-contrast image methods can capture cell differentiation over
time. (A) Neural progenitor cells demonstrate post-differentiation morphological
changes to cell bodies characteristic of neuronal phenotype and increases in
neurite outgrowth over time. (B) Accurate whole-cell segmentation extracts and

C.

Neurite length
(mm/mm2)
80
60
40

measures changes in morphological features in acquired images highlighting
cell bodies (orange) and neurites (pink). (C) Increased neurite outgrowth
post-differentiation confirms successful differentiation.

20
0

0

2
4
6
Days post-differentiation

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Sartorius eBook

Table of Contents for the Digital Edition of Sartorius eBook

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