Sartorius eBook - 34

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

Real-Time Live-Cell
Analysis of Multi-Spheroid,
Co-Cultured, 3D Tumor Assays
Kalpana Patel1, Miniver Oliver1, Nevine Holtz2,
Tim Jackson2, Nicholas Dana2, Tim Dale1, Del Trezise1
1

Sartorius, Hertfordshire, United Kingdom; 2 Sartorius, Ann Arbor, United States

Introduction

A

assay workflows. These may include fluorescent

growing body of evidence suggests

probes that perturb the biology, an end-point

studies involving microtissues and

analysis that might miss insightful temporal

organoids provide more predictive

information, or indirect biochemical readouts that

and translational observations as compared to
2D monolayer cell models1,2. Additionally, the

overlook valuable morphological insight.
The Incucyte® Live-Cell Analysis System is a

use of multicellular tumor spheroids for oncology

fully automated phase contrast and two-color

and immuno-oncology research is increasing.

fluorescence image acquisition and analysis

In vitro models that attempt to recapitulate the

platform that resides within a standard cell

tumor microenvironment may incorporate an

culture incubator for optimal cell viability and

extracellular matrix (ECM) and additional cell

maintenance of physiological relevance. The

types, such as stromal, endothelial and immune

instrument is designed to scan tissue culture

cells, allowing researchers to assess functionality

plates and flasks repeatedly over pre-determined

of drugs, evaluate chemoresistance and immune-

time intervals, enabling users to continuously

tumor cell interactions.

monitor cultures and generate quantifiable,

Current methods for assessing the growth and

kinetic information. The Incucyte® Spheroid

shrinkage of tumor spheroids are often limited

software module acts as a fast, flexible and

by time-consuming, expensive and/or laborious

powerful control hub for continuous live-cell

34 |

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