Sartorius eBook - 35

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

Vitanovski / Getty Images

analysis including image acquisition, processing,

image acquisition results in Brightfield images

and data visualization. Reagents include

with high contrast that can be readily masked

non-perturbing, cell labeling, nuclear-targeted

using built-in Incucyte® processing definitions.

GFP (green fluorescent protein), and RFP (red

Brightfield object size, count and eccentricity

fluorescent protein) for cell quantification.

are automatically plotted over time to deliver
a wealth of information on spheroid formation

Assay Principle

and growth rates. Thousands of images may

This application note describes the use of

be acquired, analyzed, and graphed, with the

the Incucyte® Live-Cell Analysis System and

capability to run up to six 96-well plates in parallel

Incucyte® 3D Multi-Tumor Spheroid Assays to

for increased throughput.

study the growth of 3D tumor multi-spheroids

Here we present validation methods and data

in co-culture with either fibroblasts or immune

that demonstrate the ability of the Incucyte®

cells, capturing data that may be missed by

Live-Cell Analysis System to kinetically visualize

single time point methods. The enhanced

and quantify the impact of stromal cells on tumor

depth of focus Brightfield (DF Brightfield)

multi-spheroid morphology and sensitivity to

image acquisition enables long-term imaging

chemotherapeutic agents, as well as to assess

of multiple tumor spheroids grown on a bed

immune cell-mediated toxicity within tumor

of extracellular matrix (Matrigel™). This superior

multi-spheroids.

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Sartorius eBook

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