Sartorius eBook - 38

THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS

Day 1

Day 3

Day 7

+ NHDF

MDA-MB-231

Day 0 (3 d post seeding)

Figure 3: Temporal effects on morphology revealed through Incucyte® DF Brightfield imaging. MDA-MB-231 cells were seeded in flat
bottom 96-well plates on a bed of Matrigel in mono- or co-culture with NHDFs (1:1 ratio, 1,000 cells/well for each) and multi-spheroids
allowed to form (3 d). Incucyte® S3 DF Brightfield images compare mono- and co-culture conditions over 7 d (10 d post cell seeding).
Note the temporal impact of NHDFs on spheroid morphology.

SK-BR-3 cells failed to form compact multi-

for each), revealed a time-dependent effect

spheroids; co-cultures with NHDFs resulted in

on spheroid morphology. MDA-MB-231 multi-

the formation of more compact aggregates

spheroids transitioned from a stellate, branched

(Figure 2). The Incucyte® Spheroid Software

appearance, evident at early time-points, to

Module permitted kinetic quantification of both

a more clustered round appearance after 6 d

morphologies, tracking multi-spheroid growth

(Figure 3).

via size measurements (Total BF Area). In addition,
different temporal growth profiles were revealed

96-Well Multi-Spheroid Growth

through live-cell analysis for a panel of four breast

and Shrinkage Assay for

tumor multi-spheroids (MCF7, MDA-MB-231,

Pharmacological Analysis

SK-BR-3, and BT-474) co-cultured with NHDFs

To demonstrate the amenability of this 3D

(Figure 2 time-course plot).

tumor and stromal cell co-culture to compound

Interestingly, NHDFs co-cultured with
MDA-MB-231 cells (1:1 ratio, 1,000 cells/well
38 |

GENengnews.com

toxicity testing, a pharmacological study was
performed using a panel of four commonly used


http://www.GENengnews.com

Sartorius eBook

Table of Contents for the Digital Edition of Sartorius eBook

Contents
Sartorius eBook - 1
Sartorius eBook - 2
Sartorius eBook - 3
Sartorius eBook - Contents
Sartorius eBook - 5
Sartorius eBook - 6
Sartorius eBook - 7
Sartorius eBook - 8
Sartorius eBook - 9
Sartorius eBook - 10
Sartorius eBook - 11
Sartorius eBook - 12
Sartorius eBook - 13
Sartorius eBook - 14
Sartorius eBook - 15
Sartorius eBook - 16
Sartorius eBook - 17
Sartorius eBook - 18
Sartorius eBook - 19
Sartorius eBook - 20
Sartorius eBook - 21
Sartorius eBook - 22
Sartorius eBook - 23
Sartorius eBook - 24
Sartorius eBook - 25
Sartorius eBook - 26
Sartorius eBook - 27
Sartorius eBook - 28
Sartorius eBook - 29
Sartorius eBook - 30
Sartorius eBook - 31
Sartorius eBook - 32
Sartorius eBook - 33
Sartorius eBook - 34
Sartorius eBook - 35
Sartorius eBook - 36
Sartorius eBook - 37
Sartorius eBook - 38
Sartorius eBook - 39
Sartorius eBook - 40
Sartorius eBook - 41
Sartorius eBook - 42
Sartorius eBook - 43
Sartorius eBook - 44
Sartorius eBook - 45
Sartorius eBook - 46
https://www.nxtbookmedia.com