Sartorius eBook - 41
THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS
MCF7
MDA-MB-231
% Control (BF area AUC)
120
% Control (BF area AUC)
120
90
90
60
60
Lapatinib
ZK164015
Camptothecin
30
-11
-10 -9
-8
-7
-6
log [compound] (M)
-5
30
-11
-10 -9
-8
-7
-6
log [compound] (M)
SK-BR-3
BT-474
% Control (BF area AUC)
120
% Control (BF area AUC)
120
90
90
60
60
30
-11
-10 -9
-8
-7
-6
log [compound] (M)
-5
30
-11
-10 -9
-8
-7
-6
log [compound] (M)
-5
-5
Figure 6: Robust and reproducible pharmacological analysis through generation of concentration response curves. A panel of four
tumor breast cell lines were seeded in 96-well flat bottom plates with NHDFs (1:1 ratio, 1,000 cells/well for each on a bed of Matrigel).
Multi-spheroids were allowed to form for 3 d prior to treatment with standard-of-care and cytotoxic agents. Concentration response
curves (CRCs) represent the area under curve (AUC) of the Total Brightfield Area (μm2) time course data (not shown) from 0-6 d (MCF7,
MDA-MB-231) or 0-10 d (SK-BR-3, BT-474) post-treatment. Each data point represents mean ± SEM, n=9-12 wells. Note: differential
compound pharmacology across cell types.
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Sartorius eBook
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