Sartorius eBook - 44
THE FUTURE OF DRUG DISCOVERY: LIVE 3D CELL ANALYSIS
Conclusions
In this application note, we demonstrate that the Incucyte® Live-Cell Analysis System, in conjunction
with the Incucyte® Spheroid Software Module, enables the analysis of 3D multi-spheroid co-cultures with
either stromal or immune cells over time and is applicable to compound testing. We have shown:
Live-cell analysis demonstrated that
Several features of the Incucyte® Live-Cell
SK-BR-3 formed more compact multi-
Analysis System are particularly advantageous for
spheroids when cultured with NHDFs
monitoring and objectively quantifying 3D multi-
Live-cell imaging revealed temporal
spheroid biology in real time. The DF Brightfield
effects of NHDFs on MDA-MB-231 multi-
imaging allows for label-free study of 3D spheroid
spheroid morphology
morphology and proliferation in 96-well assay
Cell type specific temporal growth profiles
formats for enhanced throughput. With no need
were elucidated for a panel of breast
for selection of a predefined endpoint, consistent
tumor multi-spheroids
segmentation and quantification of brightfield
The capability to perform real-time
images enables kinetic assessment of cell-
compound profiling in a 96-well format
dependent growth profiles, and the impact of
The ability to kinetically visualize and
stromal cells on tumor multi-spheroid resistance
quantify antibody-dependent cell-
to chemotherapeutic agents. Brightfield in
mediated cytotoxicity (ADCC) immune
combination with fluorescence imaging permits
cell-induced within target tumor multi-
visualization and quantification of immune cell-
spheroids in real-time
mediated toxicity of tumor multi-spheroids.
Herceptin activated PBMCs caused a
Incucyte®'s automated image acquisition,
concentration dependent loss of HER2
combined with user-friendly analysis tools and
positive multi-spheroid viability
lab tested protocols, allows non-expert users
More advanced 3D models, incorporating an
to quickly generate reproducible data, perform
extracellular matrix and additional cell types (e.g.,
analysis, and generate publication ready graphics.
stromal cells or immune cells) have the potential
Taken together, the Incucyte® Live-Cell Analysis
to provide more relevant translational models
System, Spheroid Software Module and reagents
for the study of the tumor microenvironment on
provide a unique and efficient technical platform
tumor biology.
that can be incorporated into existing workflows.
44 |
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Sartorius eBook
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