Sartorius - November 2021 - Simplifying Adeno Associated Virus - 10

How a High Throughput Bioreactor
and DOE Approach Can Help
Rapidly Optimize rAAV Production
Véronique Blouin-Tavel¹, PhD
Vector Core and Process Development
Manager
veronique.blouin@univ-nantes.fr
Magalie Penaud-Budloo¹, PhD
Co-leader of the group " Chemical
and Molecular Engineering "
Franziska Bollmann², PhD
Process Technology Consultant
Viral based Therapeutics
franziska.bollmann@sartorius.com
Christophe Chevé¹
Process Development Technical
Specialist
Marie Enga¹
Process Development Technical
Specialist
Cécile Robin¹
Process Operation Manager
Oumeya Adjali¹, MD, PhD
Laboratory Head
Amélie Boulais²
Head of Market Entry Strategy
Viral based Therapeutics
¹ INSERM UMR 1089, the University of Nantes
² Sartorius
10 | GENengnews.com
Recombinant Adeno-Associated
Virus (rAAV) is emerging as one of the
most common viral vectors for use in
gene therapy and according to the
ClinicalTrials.gov trial registry in July 2021
there were 151 active clinical studies¹ to
treat cancer or inherited diseases. However,
rAAV enabled gene therapy is still a
developing field with only a few approvals,
and therefore manufacturing is not as
well characterized as many other more
mature biological therapies. Since rAAV
is one of the main components of many
gene therapies in development there is a
need for reliable protocols to scale-up
rAAV production from small clinical trials
to larger commercial production.
Human embryonic kidney (HEK293)
and Spodoptera frugiperda (Sf9) are
the most common cell lines for rAAV production.
HEK293 cells are usually
cultured in tissue culture flasks and can
produce enough for pre-clinical and
Phase I trials. This limits the batch size
with this cell type to the number of
T-flasks which can be successfully handled
either manually or in automated systems,
making manufacturing with HEK293
cells complicated and time consuming.
These scale up challenges have led to
the need to develop suspension culture
protocols with HEK293 cells to enable
safe, cost-effective larger scale rAAV
production using stirred tank reactors
(STRs).
https://www.genengnews.com/

Sartorius - November 2021 - Simplifying Adeno Associated Virus

Table of Contents for the Digital Edition of Sartorius - November 2021 - Simplifying Adeno Associated Virus

Sartorius - November 2021 - Simplifying Adeno Associated Virus - 1
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 2
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 3
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 4
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 5
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Sartorius - November 2021 - Simplifying Adeno Associated Virus - 8
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 9
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 10
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 11
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 12
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