Sartorius - November 2021 - Simplifying Adeno Associated Virus - 13

Viability at transfection
1
0.5
-0.5
-1
-1.5
-2
Cell Line 1Cell Line 2Media 1
Media 2
Stirring Speed
pH
DO
pH × pH
Coefficients - Viability at harvest
8
6
4
2
-2
-4
Figure 2: Coefficients plots for cell viability
versus a range of cell culture factors obtained
from production of rAAV8 with suspension
HEK293 cells in Ambr®
15 micro bioreactors.
-6
Cell Line 1Cell Line 2Media 1
Media 2
Stirring Speed
pH
DO
CPPs for cell viability were determined after
transfection (above) and at harvest (below),
96 h post transfection.
13 | GENengnews.com
Viability @ harvest [%]
Viability @ transf. [%]

Sartorius - November 2021 - Simplifying Adeno Associated Virus

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Sartorius - November 2021 - Simplifying Adeno Associated Virus - 1
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 2
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 3
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 4
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 5
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 6
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 7
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 8
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 9
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Sartorius - November 2021 - Simplifying Adeno Associated Virus - 11
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 12
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 13
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 14
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 15
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 16
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 17
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 18
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 19
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 20
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 21
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 22
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 23
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 24
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 25
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 26
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 27
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 28
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 29
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 30
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 31
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