Sartorius - November 2021 - Simplifying Adeno Associated Virus - 15

4D Response Contour Plot for Specific Productivity [vg/cell]
Spec prod
[vg/cell]
Transfection reagents = TR1
3
20.000
25,000
2.5
15.000
10.000
5.000
2
20,000
Transfection reagents = TR3
1.5
15,000
1
10,000
0.5
77.1
7.27.3
pH
Dynamic Profile Plot for Titer [vg/mL]
8e +10
6e +10
4e +10
2e +10
-2e +10
6.9
6.95 7
7.05
7.1
7.15
7.2
pH
Response
L. conf. int.
7.25
7.3
7.35
7.4
7.45
0.6
0.8
Interval = 0.95 Confidence
U. conf. int.
Setpoint
pH = 6.9
1
1.2
1.4
1.6
1.8
2.0
Total Plasmid DNA [μg/mL]
Total Plasmid DNA = 3
2.2
2.4
2.6
2.8
3
7.4
77.1
7.27.3
pH
7.47.5
5,000
Target
Target
Min
Min
Figure 4: 4D response contour plot for specific productivity (vg/cell) (above) and dynamic
profile plot for titer in vg/mL (below) versus pH and total plasmid DNA as well as transfection
reagent (above only, below transfection reagent 3 only) from production of rAAV8 with
suspension HEK293 cells in Ambr®
15 micro bioreactors.
15 | GENengnews.com
Titer qPCR [vg/mL]
Total Plasmid DNA [μg/mL]
https://www.genengnews.com/

Sartorius - November 2021 - Simplifying Adeno Associated Virus

Table of Contents for the Digital Edition of Sartorius - November 2021 - Simplifying Adeno Associated Virus

Sartorius - November 2021 - Simplifying Adeno Associated Virus - 1
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 2
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 3
Sartorius - November 2021 - Simplifying Adeno Associated Virus - 4
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