Sartorius - November 2021 - Simplifying Adeno Associated Virus - 19

Future Perspectives
These studies demonstrate that using
high throughput stirred mini bioreactor
systems such as an Ambr®
15 with DOE
software MODDE®
in a step-wise
approach to determine CPPs and multifactor
optimization can be performed
quickly and efficiently.
Finding these factors and their operating
ranges can drive significant improvement
in rAAV titer for both HEK293- and Sf9based
platforms.
In our next studies we aim to scale-up
the optimized processes to a larger,
stirred bioreactor system, the Ambr®
pilot scale bioreactors.
250
Modular to test for process robustness
before we transfer the process to 50 L
Biostat STR®
Due to the costs and timelines associated
with rAAV vector production, maintaining
productivity across larger volumes by
utilizing geometrically scalable systems,
it may be possible to build a faster, more
affordable, and reliable manufacturing
process for rAAV-based gene therapies.
References
1. Clinicaltrials.gov Database Search for Clinical Trials Using AAV gene
therapy | Recruiting, Not yet recruiting, Active, not recruiting, Completed,
Enrolling by invitation Studies. Available: https://clinicaltrials.gov/ct2/results?term=AAV+gene+therapy&Search=Apply&recrs=b&recrs=a&recrs=f&rec
rs=d&recrs=e&age_v=&gndr=&type=&rslt=
2.
Jacob, A., et al. E. (2021), Homologous Recombination Offers Advantages
over Transposition-Based Systems to Generate Recombinant Baculovirus for
Adeno-Associated Viral Vector Production. Biotechnol. J., 16: 2000014.
https://doi.org/10.1002/biot.202000014
19 | GENengnews.com
https://www.doi.org/10.1002/biot.202000014 http://www.Clinicaltrials.gov https://www.clinicaltrials.gov/ct2/results?term=AAV+gene+therapy&Search=Apply&recrs=b&recrs=a&recrs=f&recrs=d&recrs=e&age_v=&gndr=&type=&rslt

Sartorius - November 2021 - Simplifying Adeno Associated Virus

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