Sartorius - November 2021 - Simplifying Adeno Associated Virus - 29

Impact of DOE and MVDA
Using a series of DOE runs in microbioreactors has helped
researchers at the University of Nantes to identify critical
process parameters (CPPs). From their DOE runs they have
identified that:
- -
-
Media 2 and Lysis Reagent 2 are beneficial
Cell density should be between 3.25 and 4.00 × 10⁶ cells/mL
Specific Baculovirus Helper | Baculovirus Vector ratio should
be within 1 and 6
In a second step, MVDA was used to evaluate a wider range
of data, by combining time series data with the DOE. Using
this technique an outlier was identified and investigated.
The result can be used to consider an appropriate scale-up
strategy with respect to oxygen supply. In summary, using
DOE and MVDA in combination with a high throughput
bioreactor mimic such as the Ambr®
15 system, has helped
researchers at the University of Nantes discover the
optimum process conditions to improve their rAAV8 titer.
Using this approach in the future could contribute to faster
process optimization and development studies for costeffectively
scaling-up their rAAV production for use in basic
research and clinical applications.
29 | GENengnews.com
https://www.genengnews.com/

Sartorius - November 2021 - Simplifying Adeno Associated Virus

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