Sartorius - November 2021 - Simplifying Adeno Associated Virus - 7

Although a DOE approach can be performed
using shake flasks, it is labor intensive,
time consuming and the results
can vary depending on who runs the experiments.
It is much faster and more accurate
to run a DOE using automated
mini bioreactors that mimic larger STRs
such as the Ambr®
15 cell culture system.
Robin explains: " It is difficult to do a DOE
in shake flasks because you cannot test
many combinations with shake flasks, as
you are limited in the number that can
be handled in parallel. But using an automated
mini bioreactor system changes
that as you can run up to 48 experiments
in parallel to characterize the CPPs more
easily and see which factors interact.
This gives us a better insight and much
faster process understanding. " BlouinTavel
adds: " Using an Ambr®
15 system
we now have the capability to screen
multiple different factors at the same
time and the screening doesn't cost as
much as we're using smaller volumes of
media and expensive reagents. We can
also test at the extreme ranges of a
factor, which is something we have not
been able to do in the past. This has led
us to developing final processes that are
up to ten-times more efficient in terms of
titer increases. "
Steps in a DOE workflow using MODDE®
software
General DOE Workflow
Step 1
Create Experimental Design
(MODDE®
)
Objective
Responses
and Factors
Design
Selection
Run Design
(Lab, Shop, floor, etc.)
Analyze Data, Create Model, Interpret
(MODDE®
)
Data
Screening
Model
Fitting
Model Result
Generation
Step 3
Step 2
Step 4: Iteration?
7 | GENengnews.com

Sartorius - November 2021 - Simplifying Adeno Associated Virus

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