Sartorisu-Sowmya eBook - 11

DECIPHERING THE IMMUNE RESPONSE TO VIRAL INFECTION

greater than the RSV vaccine canidate DS-Cav1. Behzadi
et al. (2019) repurposed the influenza A vaccine platform,
via reverse genetic engineering, to express a neutralizing
antibody against the CMV viral envelope protein (gH). In this
study, advanced flow cytometry was used to perfrom binding
analysis of viral envelope protein for the design of a new human
cytomegalovirus (CMV) vaccine. A study by Niu et al. (2019)
examined the antibody responses of convalescent patients that
had been infected with Zika virus (ZIKV), isolating three mAbs
which bound to ZIKV envolope protein that were most potent
in neutralizing the virus. Intellicyt flow cytometry was used to
perform epitope mapping of the antibodies. The antibodies
were administered to SCID mice that had been administered a
lethal dose of ZIKV and they provided protection.
These examples illustrate the value of using live-cell imaging
and analysis as well as advanced high-throughput flow
cytometry to observe viral infection, replication, and the immune
responses for the development of new therapies and vaccines.
New insights can expose viral vulnerabilities that we can use to
our advantage - and in this case, a picture is worth not just a
thousand words, but perhaps, thousands of lives. n

11 | GENengnews.com

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v11080692.
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Jul 11.
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