TTP Labtech, Phenotypic Screening - 20

PHENOTYPIC SCREENING an e-Book Series The Renaissance of Phenotypic Research

cells; similar to control-treated cells; or dissimilar
to both.
Bydefinition, high-content screening thus is
phenotypic screening, as the cells' phenotypes are
quantified. It represents the classical biological
research: Change condition and observe the
outcome, albeit at a new level of scale. Also, it
allows automated examination of more complex
subjects than cells, such as whole organisms,
tissues or even the formation of tissues and cellular
interactions over time. And finally, it inherited from
high-throughput screening the quality control and
standardization requirements for all screening
experiments: the experiment must be standardized
to optimize reproducibility at defined conditions-
typical for treated and untreated cells.
Besides its place in target-based and phenotypic
screening, high-content screening is still the
biologist's workhorse in genetic screening: All genes
of the organism under investigation (e.g. yeast) are
systematically silenced and the effect on this "gene
knockout" on the phenotype is observed using

20 | GENengnews.com

image-based high-content screening. Similarly,
high-content screening is applied in compound
combination studies to measure the effect of
combined substances on model organisms.
While there is a significant intersection between
phenotypic and high-content screening, there are
distinctions. For instance, label-free screening by
biosensors is an alternative technology, which often
can detect the smallest physiological changes in
organisms, such as the change of the center of mass
in a cell without biochemical or genetic engineering
of the cells (as would be needed to, e.g., apply an
antibody-bound dye or introduce a receptor).
Nevertheless, high-content screening is the
primary tool for all phenotypic screening today,
and most new chemical entities have been
discovered by phenotypic screening. This does
not, however, preclude target-based screening
from the drug discovery process. Target-based
screening yields more optimized entities quicker
and allows a more easy and efficient optimization
of a compound toward a target. Additionally,

the FDA requires the target of a new drug to be
identified and proven before it can be released to
the market.
All the advances in natural sciences have led to a
sophisticated set of tools and instruments to segment
and understand the effect of a given substance on
model organisms. Successful phenotypic screening
increases the mechanistic understanding of the
underlying biology. With new experimental
technologies, higher throughput, and advanced
diagnostic tools, researchers can observe phenotypes
and make a quantum leap forward in more relevant
disease models. n

Oliver Leven, Ph.D. (oliver.leven@genedata.com),
is head of professional services for the Genedata
Screener Business Unit at Genedata.


http://www.GENengnews.com

TTP Labtech, Phenotypic Screening

Table of Contents for the Digital Edition of TTP Labtech, Phenotypic Screening

Contents
TTP Labtech, Phenotypic Screening - 1
TTP Labtech, Phenotypic Screening - 2
TTP Labtech, Phenotypic Screening - 3
TTP Labtech, Phenotypic Screening - Contents
TTP Labtech, Phenotypic Screening - 5
TTP Labtech, Phenotypic Screening - 6
TTP Labtech, Phenotypic Screening - 7
TTP Labtech, Phenotypic Screening - 8
TTP Labtech, Phenotypic Screening - 9
TTP Labtech, Phenotypic Screening - 10
TTP Labtech, Phenotypic Screening - 11
TTP Labtech, Phenotypic Screening - 12
TTP Labtech, Phenotypic Screening - 13
TTP Labtech, Phenotypic Screening - 14
TTP Labtech, Phenotypic Screening - 15
TTP Labtech, Phenotypic Screening - 16
TTP Labtech, Phenotypic Screening - 17
TTP Labtech, Phenotypic Screening - 18
TTP Labtech, Phenotypic Screening - 19
TTP Labtech, Phenotypic Screening - 20
TTP Labtech, Phenotypic Screening - 21
TTP Labtech, Phenotypic Screening - 22
TTP Labtech, Phenotypic Screening - 23
TTP Labtech, Phenotypic Screening - 24
TTP Labtech, Phenotypic Screening - 25
TTP Labtech, Phenotypic Screening - 26
TTP Labtech, Phenotypic Screening - 27
TTP Labtech, Phenotypic Screening - 28
https://www.nxtbook.com/nxtbooks/gen/ttplabtech_phenotypicscreening
https://www.nxtbookmedia.com