TTP Labtech, Phenotypic Screening - 22

PHENOTYPIC SCREENING an e-Book Series Novel 3D Cell Culture Systems

One clear example he cited is the response of tumor cells to drug therapy and
the fact that tumor cells grown in 2D versus 3D cultures tend to respond quite
differently to the same concentration of an antitumor drug. Tumor cells grown
in 3D are typically more resistant to therapeutic compounds.
When cells grow in 3D spherical clusters, compared to 2D monolayers, the
level of oxygenation differs depending on the whether a cell is situated more
toward the inside or the outside of a spheroid structure. The difference in level of
oxygenation appears to influence the response to drug therapy, explained Dr. Pardo.
Additionally, differences in the extracellular matrix produced by cells growing in
3D culture systems can modify the cellular response to drugs and other stimuli,
likely because of changes in integrin-based signaling in 3D compared to 2D cell
cultures.
Another factor that can affect the therapeutic drug response of cells grown in
culture is the extent of cell-to-cell contacts, which will tend to be more developed
in 3D environments with higher cell densities.
3D culture systems are especially useful for studying the invasive properties
and metastatic potential of tumor cells and for conducting screening assays
for cell migration. In his presentation, Dr. Pardo described screens of large
Olivier Pardo and colleagues at the Cellular Regulatory Networks Group, Imperial
College, London, use a combination of 2D and 3D cell culture assays and whole
zebrafish models to study the invasiveness and metastatic potential of tumor
cells. This image depicts green fluorescent protein (GFP)-labeled lung cancer cells
growing in a zebrafish embryo, a model system used to study metastasis in vivo.
22 | GENengnews.com


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TTP Labtech, Phenotypic Screening

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