TTP Labtech, Phenotypic Screening - 23

PHENOTYPIC SCREENING an e-Book Series Novel 3D Cell Culture Systems

compound libraries using a bone metastatis
assay or extra/intravasation assay, small-tomedium library screens with a 3D collagen
invasion assay, and screening of a small number
of targets using a zebrafish metastatic model
organism. The 3D collagen invasion assay with
confocal image acquisition was performed
in 96-microwell plates.
Whereas manual screening was slow and
difficult, making this assay format ill-suited for
large library screening, the results obtained were
highly reproducible, with >90% overlap between
repeat results. In contrast, automating each step
of the assay-and providing temperature control
to minimize collagen polymerization-increased
the throughput of the assay to make it suitable for
large-scale screening, but the reproducibility of the
robotized screen was poor, with <33% overlap
between repeat results.
Limitations related to automation and
the cost of most 3D cell culture technologies and
materials available today are two important factors
that stand in the way of using these methods for
23 | GENengnews.com

96-well microtissue assay plate (InSphero's GravityTRAP™) loaded with tumor microtissues composed of the
colon cancer cell line HCT116 (left) expressing a green fluorescent protein and stromal mouse fibroblasts NIH3T3
expressing a red fluorescent protein. Visualization of the individual cell populations using the PerkinElmer
Opera QEHS equipped with a 10x Air objective. The single z-stack through the microtissue was processed with
the Acapella High Content Imaging and Analysis Software to generate a maximum projection image (right).
(Image provided by PerkinElmer, Karin Boettcher, and Stefan Letzsch)


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TTP Labtech, Phenotypic Screening

Table of Contents for the Digital Edition of TTP Labtech, Phenotypic Screening

Contents
TTP Labtech, Phenotypic Screening - 1
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